Recombinant Human EED Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1704
Recombinant Human EED Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1704
Catalog No.: BLPSN-1704
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | O75530 |
| Synonym | HEED, WAIT1 |
| Background | EED is essential for regulating the repressive histone modification, histone 3 lysine 27 tri-methylation (H3K27me3) at many developmental genes. EED depletion significantly impeded erythroid maturation. EED depletion impaired the generation of hematopoietic stem cells. The function of EED within polycomb repressive complex 2 (PRC2) is mediated by a complex network of protein-protein interactions. The targeted disruption of EED's reader function can lead to allosteric inhibition of PRC2 catalytic activity. Eed(Delta/Delta) HSPCs exhibited increased attachment to a major extracellular matrix component, fibronectin. Thus, EED deficiency increases proliferation on one side but promotes quiescence possibly by enhanced adhesion to the hematopoietic niche on the other, and these conflicting events would lead to abnormal differentiation and functional defect of Eed(Delta/Delta) HSPCs. |
| Description | A DNA sequence encoding the human EED (O75530-1) (Met 1-Arg 441) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met 1-Arg 441 |
| Molecular Weight | The recombinant human EED/GST chimera consists of 678 a.a. and has a calculated molecular mass of 78 kDa. It migrates as an approximately 75-85 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >93% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 2mM GSH, 10% gly. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
