Recombinant Human EDAR Protein

Name: Recombinant Human EDAR Protein
Brand: Beta LifeScience
SKU: BL-3101PS
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Tag	N/A
Host Species	Human
Synonym	Ectodysplasin A Receptor, DL, Ectodysplasin 1, AnhidroticReceptor, Anhidrotic Ectodysplasin Receptor 1, Ectodermal Dysplasia Receptor,Ectodysplasin-A Receptor, Downless Homolog, EDA-A1 Receptor, ECTD10A, ECTD10B, EDA3,HRM1, ED3, Tumor Necrosis Factor Receptor Superfamily Member EDAR, Downless,Mouse, Homolog Of, EDA-A1R, EDA1R, ED1R, ED5, Tumor necrosis factor receptorsuperfamily member EDAR.
Background	Ectodysplasin A Receptor, also known as EDAR belongs to the tumornecrosis factor receptor family. EDAR isa receptor for the soluble ligand ectodysplasin A, and is capable of activatingthe nuclear factor-kappaB, JNK, as well as caspase-independent cell deathpathways. EDAR is necessary for the development of hair, teeth, and otherectodermal derivatives. Furthermore, mutations in EDAR resulted in autosomaldominant and recessive forms of hypohidrotic ectodermal dysplasia.
Description	EDAR Human Recombinant expressed in E.Coli is a single,non-glycosylated polypeptide chain containing 445a.a. (27-448 a.a) andhaving a molecular weight of 48.2kDa.EDAR is fused to a 23a.a. His-tag at N-terminusand purified by unique purification methods.
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MGSEYSNCGE NEYYNQTTGL CQECPPCGPG EEPYLSCGYG TKDEDYGCVPCPAEKFSKGG YQICRRHKDC EGFFRATVLT PGDMENDAEC GPCLPGYYML ENRPRNIYGM VCYSCLLAPPNTKECVGATS GASANFPGTS GSSTLSPFQH AHKELSGQGHLATALIIAMS TIFIMAIAIV LIIMFYILKT KPSAPACCTS HPGKSVEAQVSKDEEKKEAP DNVVMFSEKD EFEKLTATPA KPTKSENDAS SENEQLLSRS VDSDEEPAPD KQGSPELCLLSLVHLAREKS ATSNKSAGIQ SRRKKILDVY ANVCGVVEGL SPTELPFDCL EKTSRMLSSTYNSEKAVVKT WRHLAESFGL KRDEIGGMTD GMQLFDRIST AGYSIPELLTKLVQIERLDA VESLCADILE WAGVVPPASQ PHAAS.
Purity	>85.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	EDAR protein solution (0.25mg/ml) containing 20mMTris-HCl (pH 8.0) and 10% glycerol.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.