Recombinant Human ECE2 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1685
Recombinant Human ECE2 Protein (Fc Tag)

Recombinant Human ECE2 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1685
Catalog No.: BLPSN-1685

Product Overview

Tag Fc
Host Species Human
Accession NP_055508.3
Background Endothelin-converting enzyme 2, also known as ECE-2, is a metalloprotease that possesses many properties consistent with it being a neuropeptide-processing enzyme. Endothelin-converting enzymes (ECEs) are the key enzymes in the endothelin (ET) biosynthesis that catalyze the conversion of big ET, the biologically inactive precursor of mature ET. Two enzymes, termed ECE-1 and ECE-2, have been molecularly identified. ECE-2 is found primarily in neural tissues, with high levels of expression in midbrain, cerebellum, hypothalamus, frontal cortex and spinal cord and moderate levels in hippocampus and striatum. ECE-2 is strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level). ECE-2 converts big endothelin-1 to endothelin-1. It is involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. ECE-2 may limit beta-amyloid peptide accumulation in brain. It may also have methyltransferase activity. A comparison of residues around the cleavage site revealed that ECE-2 exhibits a unique cleavage site selectivity that is related to but distinct from that of ECE-1.
Description A DNA sequence encoding the ectodomain of human endothelin converting enzyme 2 isoform A (NP_055508.3) (Gly 199-Trp 883) was fused with the Fc region of human IgG1 at the N-terminus.
Source HEK293
Predicted N Terminal Glu 20
AA Sequence Gly 199-Trp 883
Molecular Weight The recombinant human Fc/ECE2 is a disulfide-linked homodimeric protein. The reduced monomer consists of 921 a.a. and predicts a molecular mass of 105 kDa. As a result of glycosylation, the apparent molecular mass of rh ECE2/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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