Recombinant Human E-Cadherin / ARC1 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1677

Recombinant Human E-Cadherin / ARC1 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1677
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Product Overview

Tag Fc
Host Species Human
Accession P12830
Synonym Arc-1, CD324, CDH1, CDHE, E-cad, E-Cadherin, ECAD, LCAM, UVO
Background Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
Description A DNA sequence encoding the human E-Cad (P12830)(Met1-Ile707) was expressed with the Fc region of human IgG1 at the C-terminus.
Source HEK293
Predicted N Terminal Gln 23 & Asp 155
AA Sequence Met1-Ile707
Molecular Weight The recombinant human E-Cad/Fc is a disulfide-linked homodimer. The reduced monomer comprises 791 a.a. and has a predicted molecular mass of 87.1 kDa. The apparent molecular mass of the protein is approximately 116-126 kDa in SDS-PAGE under reducing conditions.
Purity >90% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells.When cells are added to E-Cad coated plates (5 ug/mL, 100 uL/well), approximately 33% will adhere specifically after 90 minutes at 37 °C.
Formulation Lyophilized from sterile PBS, pH 7.4..
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.; E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.; (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.
Subcellular Location Cell junction, adherens junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
Database References
Associated Diseases Hereditary diffuse gastric cancer (HDGC); Endometrial cancer (ENDMC); Ovarian cancer (OC); Breast cancer, lobular (LBC); Blepharocheilodontic syndrome 1 (BCDS1)
Tissue Specificity Non-neural epithelial tissues.

Gene Functions References

  1. pathogenic variants are described in four genes encoding components of the p120-catenin complex (CTNND1, PLEKHA7, PLEKHA5) and an epithelial splicing regulator (ESRP2), in addition to the known Cleft lip/Palate-associated gene, CDH1, which encodes E-cadherin. PMID: 29805042
  2. NEDD9, E-cadherin and gamma-catenin proteins have roles in pancreatic ductal adenocarcinoma PMID: 29924959
  3. detection of Ezrin and E-cadherin expression in cervical smears, could be a potential prognostic marker for identifying cervical lesions with high-risk of progression to invasive cervical cancer, and may help on the selection of an appropriate therapy or avoid unnecessary treatment PMID: 29587669
  4. CDH1 plays an essential role in epithelial cell adherence. Causative of blepharocheilodontic syndrome CDH1 mutations impair the cell adhesion function of the cadherin-catenin complex in a dominant-negative manner. PMID: 29348693
  5. Together, these data suggest that the S18-2 protein induces epithelial to mesenchymal cell transition through the TWIST2/E-cadherin signalling and, consequently, CXCR4-mediated migration of prostate cancer cells. PMID: 29396484
  6. In the present study, we demonstrated that miR711mediated downregulation of CD44 expression inhibited EMT of gastric cancer cells in vitro and in vivo by downregulating vimentin protein expression and upregulating Ecadherin protein expression through transfection, qRTPCR and western blotting PMID: 30226620
  7. Soluble E-cadherin (sE-cad) (an 80-kDa soluble form), which is highly expressed in the malignant ascites of ovarian cancer patients, is a potent inducer of angiogenesis. In addition to ectodomain shedding, we provide further evidence that sE-cad is abundantly released in the form of exosomes. PMID: 29891938
  8. In the former, p53 binds to the CDH1 (encoding E-cadherin) locus to antagonize EZH2-mediated H3K27 trimethylation (H3K27me3) to maintain high levels of acetylation of H3K27 (H3K27ac). PMID: 29371630
  9. E-cadherin silencing relies on the formation of a complex between the paRNA and microRNA-guided Argonaute 1 that, together, recruit SUV39H1 and induce repressive chromatin modifications in the gene promoter PMID: 28555645
  10. The results show how E-cadherin instructs the assembly of the LGN/NuMA complex at cell-cell contacts, and define a mechanism that couples cell division orientation to intercellular adhesion. PMID: 28045117
  11. Low CDH1 expression is associated with pancreatic cancer. PMID: 29956814
  12. the dysregulation of TET2/E-cadherin/beta-catenin regulatory loop is a critical oncogenic event in HCC progression PMID: 29331390
  13. At the molecular level, transcription of the adherens junction protein E-cadherin is upregulated on nicotinic acid addition, leading to accumulation of E-cadherin protein at the cell-cell boundary. This can be attributed to nicotinic acid's ability to facilitate the ubiquitination and degradation of Snail1, a transcription factor that represses E-cadherin expression. PMID: 28256591
  14. down-regulation of USP48 increases E-cadherin expression and epithelial barrier integrity through reducing TRAF2 stability PMID: 28874458
  15. AnxA5 2D-network mediates E-cadherin mobility in the plasmalemma that triggers human trophoblasts aggregation and thereby cell fusion. PMID: 28176826
  16. The disassociation of the beta-catenin/E-cadherin complex in the osteoblast membrane under stretch loading and the subsequent translocation of beta-catenin into the nucleus may be an intrinsic mechanical signal transduction mechanism. PMID: 29901167
  17. The presence of E-cadherin decreases cortical contractility during mitosis through a signaling cascade leading to multipolar divisions, and its knockout promotes clustering and survival of cells with multiple centrosomes. PMID: 29133484
  18. E-cadherin expression is not significantly linked to metastatic disease in pancreatic ductal adenocarcinoma. PMID: 29355490
  19. High CDH1 expression is associated with the Pathogenesis of Adamantinomatous Craniopharyngiomas. PMID: 29625497
  20. study provides evidence for genetic polymorphisms of the adherent junction component cadherin gene and the association of its haplotypes with leukoaraiosis PMID: 30017735
  21. found that E-cadherin, N-cadherin and fibronetin are involved in CHD4-mediated epithelial-mesenchymal transition PMID: 29305962
  22. Up-regulation of H19 in bladder cancer tissues is correlated with clinical stage or metastasis of cancer. By cell transfection to suppress H19 expression in bladder cancer cells, E-cadherin expression is up-regulated, thus weakening metastatic potency of cancer cells. PMID: 29614625
  23. These findings suggested that PHF8 played an oncogenic role in facilitating FIP200-dependent autophagic degradation of E-cadherin, EMT and metastasis in hepatocellular carcinoma (HCC). PHF8 might be a promising target for prevention, treatment and prognostic prediction of HCC. PMID: 30180906
  24. When ANXA5 expression increased, cell proliferation was inhibited by regulating the expression of bcl-2 and bax while cell metastasis was suppressed by regulating E-cadherin and MMP-9 expression. PMID: 30010106
  25. Six2 is negatively correlated with good prognosis and decreases 5-FU sensitivity via suppressing E-cadherin expression in HCC cells. PMID: 29772441
  26. The - 73A > C CDH1 promoter variation may lead to differences in the overall survival of sporadic gastric carcinoma patients and allele-specific repressions of CDH1. PMID: 29168119
  27. Overexpression of KLF6-SV1 is associated with young patients, and loss of E-cadherin suggests that this variant correlated with the aggressiveness of nasopharyngeal carcinoma. PMID: 29854578
  28. Smad4 could be considered as a central component of EMT transition in human colorectal cancer that combines with transcriptional factors to reduce E-cadherin and alter the expression of the epithelial phenotype. PMID: 29468299
  29. hnRNP H/F are important for maintenance and differentiation of embryonic stem cells and that this at least in part reflects a switch in TCF3 alternative splicing that leads to repression of CDH1/E-cadherin. PMID: 30115631
  30. E-Cadherin and epithelial syndecan-1 were more highly expressed in intraluminal/luminal unicystic ameloblastoma than in mural unicystic ameloblastoma and solid/multicystic ameloblastoma, whereas the stromal expression of syndecan-1 was higher in mural unicystic ameloblastoma and solid/multicystic ameloblastoma. PMID: 29850393
  31. miR-219-5p promotes tumor growth and metastasis of HCC by regulating CDH1 and can serve as a prognostic marker for HCC patients. PMID: 29862272
  32. Plasma sE-cadherin levels and sE-cadherin/sVE-cadherin ratios are potential biomarkers for COPD. PMID: 29376431
  33. the HDAC inhibitors augmented both Ecadherin and vimentin expression and their effects varied in different cholangiocarcinoma cell lines. Therefore, the clinical use of HDAC inhibitors in biliary cancer should be considered cautiously PMID: 29767267
  34. E-cadherin expression was preserved in 10 (21.28%) of the 47 NSCLCs immunostained with anti-E-cadherin antibody and reduced/absent in 37 of the 47 (78.72%) NSCLCs studied. E-cadherin plays a major role in the intercellular adhesion. PMID: 29556623
  35. CDH1 promoter methylation may be correlated with cervical cancer carcinogenesis, especially for Caucasians. It was associated with histological subtypes PMID: 29237293
  36. High UTX expression is independently associated with a better prognosis in patients with esophageal squamous cell carcinoma (ESCC) and downregulation of UTX increases ESCC cell growth and decreases E-cadherin expression. Our results suggest that UTX may be a novel therapeutic target for patients with ESCC. PMID: 29351209
  37. Data suggest that ECAD, STAT3, Bak, and Bcl-xL are expressed in affected endometrial tissues of women with endometrioid adenocarcinoma depending on neoplasm staging and cell differentiation. This study was conducted using immunohistochemistry of surgically resected tissues. (STAT3 = signal transducer and activator of transcription 3 protein; Bak = pro-apoptotic protein BAK; Bcl-xL = BCL2 associated agonist of cell death) PMID: 28937296
  38. LncRNA RP11-789C1.1 inhibited EMT in GC through the RP11-789C1.1/miR-5003/E-cadherin axis, which could be a promising therapeutic target for Gastric Cancer. PMID: 29991048
  39. Using single-molecule localization microscopy, we show that pAJs in these cells reach more than 1 mum in length and consist of several cadherin clusters with crystal-like density interspersed within sparser cadherin regions. Notably, extrajunctional cadherin appears to be monomeric, and its density is almost four orders of magnitude less than observed in the pAJ regions. PMID: 29691319
  40. CDH1 methylation may play a role in the initiation and progression of salivary carcinoma ex pleomorphic adenoma PMID: 29207084
  41. We illustrate the approach using immunohistochemical measurements of the epithelial-mesenchymal transition marker E-cadherin in a set of colorectal primary tumors from a population-based prospective cohort in North Carolina PMID: 29338703
  42. The aim of our study was to analyze the immunohistochemical expression of beta-catenin, E-cadherin and Snail, depending on clinico-morphological aspects of the laryngeal squamous cell carcinomas. Results revealed variable E-cadherin, beta-catenin and Snail expression, depending on differentiation degree and tumor stage. PMID: 29250652
  43. Twist, E-cadherin, and N-cadherin protein were differently expressed in endometrioid adenocarcinoma tissues and in normal endometrium which indicates their potential function for endometrioid adenocarcinoma development. PMID: 29237910
  44. Findings uncover a new regulatory network in RCC involving metastasis-promoting miR-720 that directly targets expression of key metastasis-suppressing proteins E-cadherin and alphaE-catenin complex. PMID: 28802251
  45. Results show that E-cadherin expression levels were negatively regulated by 90K via ubiquitination-mediated proteasomal degradation in a cell density-dependent manner. PMID: 29207493
  46. these results indicate that increased alpha-actinin-1 expression destabilizes E-cadherin-based adhesions, which is likely to promote the migratory potential of breast cancer cells. Furthermore, our results identify a-actinin-1 as a candidate prognostic biomarker in basal-like breast cancer. PMID: 29742177
  47. High glucose enhances the formation of EZH2/Snail/HDAC1 complex in the nucleus, which in turn causes E-cadherin repression. PMID: 29705809
  48. TGF-beta1 induced epithelial-mesenchymal transition in non-small cell lung cancer cells by upregulating miR-9 and downregulating miR-9's target, E-cadherin. PMID: 29118814
  49. Results show that E-cadherin/beta-catenin complex is disrupted by ICAT promoting epithelial-mesenchymal transition of cervical cancer cells. . PMID: 29048651
  50. Studies categorize cadherin 1 (CDH1) variants, as neutral or deleterious. PMID: 29231860

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