Recombinant Human DYRK3 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1671
Recombinant Human DYRK3 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1671
Catalog No.: BLPSN-1671
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | O43781 |
| Synonym | DYRK5, hYAK3-2, RED, REDK |
| Background | Dual specificity tyrosine-phosphorylation-regulated kinase 3, also known as Regulatory erythroid kinase, REDK and DYRK3, is a nucleus protein which belongs to theprotein kinase superfamily, CMGC Ser/Thr protein kinase family and MNB/DYRK subfamily. DYRKs are an emerging family of dual-specificity kinases that play key roles in cell proliferation, survival, and development. DYRK3 contains oneprotein kinase domain. Isoform 1 and isoform 2 of DYRK3 are highly expressed in testis and in hematopoietic tissue such as fetal liver, and bone marrow. Isoform 2 of DYRK3 is the predominant form in testis. Isoform 1 of DYRK3 is the predominant form in fetal liver and bone marrow. Isoform 1 and isoform 2 are present at low levels in heart, pancreas, lymph node, and thymus. DYRK3 is a negative regulator of EPO-dependent erythropoiesis. It may place an upper limit on red cell production during stress erythropoiesis. DYRK3 inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells. It may also act by regulating CREB/CRE signaling. DYRK3 proved to effectively inhibit NFAT (nuclear factor of activated T cells) transcriptional response pathways and to co-immunoprecipitate with NFATc3. DYRK3 attenuates (and possibly apportions) red cell production selectively during anemia. |
| Description | A DNA sequence encoding the human DYRK3 isoform 1 (O43781-1) (Met 1-Ser 588) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met 1-Ser 588 |
| Molecular Weight | The recombinant human DYRK3/GST chimera consists of 825 a.a. and has a calculated molecular mass of 93.5 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | The specific activity was determined to be 22 nmol/min/mg using synthetic DYRKtide peptide (RRRFRPASPLRGPPK) as substrate. |
| Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, 0.5mM PMSF, 10% gly, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
