Recombinant Human DUSP14 Protein (His & MBP Tag)

Beta LifeScience SKU/CAT #: BLPSN-1667
Recombinant Human DUSP14 Protein (His & MBP Tag)

Recombinant Human DUSP14 Protein (His & MBP Tag)

Beta LifeScience SKU/CAT #: BLPSN-1667
Catalog No.: BLPSN-1667

Product Overview

Tag His&MBP
Host Species Human
Accession O95147
Synonym MKP-L, MKP6
Background Dual specific phosphatase 14 / MAP-kinase phophatase-6 (DUSP14 / MKP6) is a member of Dual-specificity phosphatases that is a subclass of protein tyrosine phosphatases (PTP) families that can dephosphorylate bothe phosphotyrosine and phosphoserine / phosphothreonine residues in substrates. Unlike many other DUSPs, DUSP14 only contains a catalytic domain within the C-terminal region. In signal transduction, DUSP14 has been considered as negative regulator of the mitogen-activated protein kinase (MAPK) / extracellular signal-regulated kinase 1 / 2 (ERK 1 / 2) pathway. DUSP14 phosphatase activity has been confirmed to be inhibited by PTP inhibitor -…£. PTP inhibitor binds to the catalytic site of DUSP14. PTP inhibitor -…£ effectively and specifically inhibited DUSP14-mediated dephosphorylation of JNK, a member of the mitogen-activated protein kinase (MAPK) family through dephosphorylation of both the Ser / Thr and Tyr residues of MAPKs.
Description A DNA sequence encoding the human DUSP14 (O95147) (Met 1 -His 191) was fused with an N-terminal His-tagged MBP tag at the N-terminus.
Source E.coli
Predicted N Terminal Met
AA Sequence Met 1 -His 191
Molecular Weight The recombinant human DUSP14/MBP fusion protein consists of 587 a.a. and has a calculated molecular mass of 65 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
Purity >88% as determined by SDS-PAGE
Endotoxin Please contact us for more information.
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.5.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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