Recombinant Human DUSP14 Protein (His & MBP Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1667
Recombinant Human DUSP14 Protein (His & MBP Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1667
Catalog No.: BLPSN-1667
Product Overview
| Tag | His&MBP |
| Host Species | Human |
| Accession | O95147 |
| Synonym | MKP-L, MKP6 |
| Background | Dual specific phosphatase 14 / MAP-kinase phophatase-6 (DUSP14 / MKP6) is a member of Dual-specificity phosphatases that is a subclass of protein tyrosine phosphatases (PTP) families that can dephosphorylate bothe phosphotyrosine and phosphoserine / phosphothreonine residues in substrates. Unlike many other DUSPs, DUSP14 only contains a catalytic domain within the C-terminal region. In signal transduction, DUSP14 has been considered as negative regulator of the mitogen-activated protein kinase (MAPK) / extracellular signal-regulated kinase 1 / 2 (ERK 1 / 2) pathway. DUSP14 phosphatase activity has been confirmed to be inhibited by PTP inhibitor -…£. PTP inhibitor binds to the catalytic site of DUSP14. PTP inhibitor -…£ effectively and specifically inhibited DUSP14-mediated dephosphorylation of JNK, a member of the mitogen-activated protein kinase (MAPK) family through dephosphorylation of both the Ser / Thr and Tyr residues of MAPKs. |
| Description | A DNA sequence encoding the human DUSP14 (O95147) (Met 1 -His 191) was fused with an N-terminal His-tagged MBP tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | Met |
| AA Sequence | Met 1 -His 191 |
| Molecular Weight | The recombinant human DUSP14/MBP fusion protein consists of 587 a.a. and has a calculated molecular mass of 65 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >88% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.5. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
