Recombinant Human DUSP14 Protein (His & MBP Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1667
Recombinant Human DUSP14 Protein (His & MBP Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1667
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His&MBP |
Host Species | Human |
Accession | O95147 |
Synonym | MKP-L, MKP6 |
Background | Dual specific phosphatase 14 / MAP-kinase phophatase-6 (DUSP14 / MKP6) is a member of Dual-specificity phosphatases that is a subclass of protein tyrosine phosphatases (PTP) families that can dephosphorylate bothe phosphotyrosine and phosphoserine / phosphothreonine residues in substrates. Unlike many other DUSPs, DUSP14 only contains a catalytic domain within the C-terminal region. In signal transduction, DUSP14 has been considered as negative regulator of the mitogen-activated protein kinase (MAPK) / extracellular signal-regulated kinase 1 / 2 (ERK 1 / 2) pathway. DUSP14 phosphatase activity has been confirmed to be inhibited by PTP inhibitor -…£. PTP inhibitor binds to the catalytic site of DUSP14. PTP inhibitor -…£ effectively and specifically inhibited DUSP14-mediated dephosphorylation of JNK, a member of the mitogen-activated protein kinase (MAPK) family through dephosphorylation of both the Ser / Thr and Tyr residues of MAPKs. |
Description | A DNA sequence encoding the human DUSP14 (O95147) (Met 1 -His 191) was fused with an N-terminal His-tagged MBP tag at the N-terminus. |
Source | E.coli |
Predicted N Terminal | Met |
AA Sequence | Met 1 -His 191 |
Molecular Weight | The recombinant human DUSP14/MBP fusion protein consists of 587 a.a. and has a calculated molecular mass of 65 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions. |
Purity | >88% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.5. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Involved in the inactivation of MAP kinases. Dephosphorylates ERK, JNK and p38 MAP-kinases. |
Protein Families | Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily |
Database References |
Gene Functions References
- Hepatocyte DUSP14 is required for maintaining hepatic metabolic homeostasis and for suppressing inflammation, a novel function that relies on constraining TAK1 hyperactivation PMID: 29077210
- The AA genotype was associated with protection against active TB. Among disease-free individuals, T-helper type-1 related genes, IFNGR2 and STAT1 mRNA levels significantly increased as the number of A alleles of rs1051838 increased. PMID: 26938665
- levels significantly decreased in failing hearts PMID: 26891723
- these findings reveal a novel mechanism by which TRAF2 mediates Lys63-linked ubiquitination of DUSP14, leading to DUSP14 activation in T cells PMID: 26521044
- findings suggest that DUSP14 negatively regulates TNF- or IL-1-induced NF-kappaB activation by dephosphorylating TAK1 at Thr-187 PMID: 23229544
- DUSP14 may be a susceptibility gene for pulmonary tuberculosis. PMID: 22233810
- The overproduction, purification and crystal structure at 1.88 A resolution of human dual-specificity phosphatase 14, DUSP14 (MKP6), are reported. PMID: 19770498
- Activation of ATM by radiation down-regulates phospho-ERK1/2 and its downstream signaling via increased expression of mitogen-activated protein kinase phosphatase MKP-1. PMID: 17178844
- The binding of Lipomannan to TLR2 triggers MAPK activation, followed by an up-regulation of MKP-1 expression, which in turn may act as a negative regulator of MAPK activation. PMID: 18201568