Recombinant Human Dopamine beta-Hydroxylase / DBM Protein (His Tag)

Name: Recombinant Human Dopamine beta-Hydroxylase / DBM Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1638
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	P09172
Synonym	DBM
Background	DBH is a 29 kDa copper-containing oxygenase. It can be detected in noradrenergic nerve terminals of the central and peripheral nervous systems, and is also expressed in chromaffin cells of the adrenal medulla. DBH contains our identical subunits, and its activity requires ascorbate as a cofactor. It functions in in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine the only transmitter synthesized inside vesicles. DBH has been shown to be associated with decision making and addictive behaviors such as alcohol and smoking, attention deficit hyperactivity disorder, and also with neurological diseases such as Schizophrenia and Alzheimer's.
Description	A DNA sequence encoding the human DBH (P09172) (Ser26-Gly603) was expressed with an N-terminal His tag.
Source	HEK293
Predicted N Terminal	His
AA Sequence	Ser26-Gly603
Molecular Weight	The recombinant human DBH comprises 598 a.a. and has a predicted molecular mass of 67.3 kDa. The apparent molecular mass of the protein is approximately 68 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Conversion of dopamine to noradrenaline.
Subcellular Location	[Soluble dopamine beta-hydroxylase]: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Secreted.; Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein.
Protein Families	Copper type II ascorbate-dependent monooxygenase family
Database References	HGNC: 2689 OMIM: 223360 KEGG: hsa:1621 STRING: 9606.ENSP00000376776 UniGene: Hs.591890
Associated Diseases	Dopamine beta-hydroxylase deficiency (DBH deficiency)

Gene Functions References

Most DBH polymorphisms from the currently available loci showed no linkage to Alzheimer's disease, Parkinson's disease, and schizophrenia, indicating the lower possibility of these loci serving as genetic markers of the risks of diseases with neurodegenerative characteristics. On the other hand, the DBH rs2283123 and rs2007153 polymorphisms could have opposite effects on schizophrenia development in Caucasians PMID: 30453293
The findings of this study confirmed a strong association between genotype at rs1611115 and pDbetaH activity in Chinese patients with schizophrenia. Our data also suggest the rs1108580 polymorphism may influence some aspects of cognitive function in schizophrenia. PMID: 28647493
Its single-nucleotide polymorphisms involves in dopaminergic metabolism and motor and cognitive function in older adults PMID: 29525179
This study demonstrate the genetic influence of a family history of alcohol use disorders and DAT and DBH gene polymorphisms on the risk of withdrawal seizures and delirium tremens. PMID: 28139629
Homospecific activity computed for the WT of DBH and variant proteins showed a marginal decrease in A318S, W544S and R549C variants PMID: 28707163
This study indicated that DBH5'-Ins/Del polymorphism may not play a role in the susceptibility to tardive dyskinesia and cognitive deficits in schizophrenia with tardive dyskinesia. PMID: 27776953
DRD2 A2/A1, DRD3 Ser9Gly, DbetaH -1021C>T, OPRM1 A118G and GRIK1 rs2832407C>A are not associated with alcoholism alone or in interaction. PMID: 27447243
Results suggest that interference of cannabis and cocaine with cognitive impulse control and functional corticostriatal connectivity depends on DBH genotype. PMID: 26667034
Dopamine beta-Hydroxylase Deficiency is associated with Hyperinsulinemia and Insulin Resistance. PMID: 27778639
Dopamin Beta Hydroxylase gene +1603C > T polymorphism may be one of the many genetic factors for migraine susceptibility in the Turkish population. PMID: 26868704
The genotype and allele distribution frequencies in rs1611115 were different between Parkinson's disease patients and the healthy control. The TT genotype may lead to a 2.95 times higher risk of Parkinson's disease occurrence compared with the common genotype CC. DBH rs1611115 polymorphism was likely to be associated with the susceptibility to PD, but we did not find that rs732833 is a susceptibility marker PMID: 27177268
Results suggest that the DBH gene may play an important role in the occurrence of schizophrenia (SCZ). Also, rs1611114 may be associated with SCZ susceptibility and related clinical symptoms in the Chinese Zhuang but not Han Chinese population. PMID: 27236774
Study found that the functional T allele in DBH SNP rs1611115, which reduces the conversion of dopamine to norepinephrine, corresponds with different BOLD-signal changes in responses to gambling, drug or sad cues in individuals with and without pathological gambling PMID: 27194378
This study demonstrated that the association between DbetaH genotype (C1021T), early onset of conduct disorder and psychopathic traits in juvenile delinquents. PMID: 26616837
We report the crystal structure of human dopamine b-hydroxylase, which is the enzyme converting dopamine to norepinephrine. The structure of the DOMON (dopamine b-monooxygenase N-terminal) domain, also found in 1600 other proteins, reveals a possible metal-binding site and a ligand-binding pocket PMID: 27152332
The p.Val26Met variant in the dopamine beta-hydroxylase gene at 9q34.2 was associated with Lung Cancer. PMID: 26762739
A DBH gene variant, rs129882, which confers risk to attention deficit hyperactivity disorder, is also associated with reduced in vitro gene expression. PMID: 25975715
No significant association was found between the DbetaH 5'-Ins/Del polymorphism and patients with chronic schizophrenia. PMID: 26891013
This study finding significant excess of rare nonsynonymous variants exclusive to European Americans smokers in DBH. PMID: 25450229
did not find a significant association of rs1611115 with PTSD risk [meta-analysis] PMID: 26986136
Reported is the construction of a tissue engineered intervertebral disc with high biological activity using an allogeneic intervertebral disc supplemented with transfected nucleus pulposus cells expressing exogenous dopamine beta-hydroxylase. PMID: 26400296
There was no significant relationship between Migraine susceptibility and genetic polymorphisms of DBH. PMID: 26632697
Results suggest that the DBH5'-Ins/Del polymorphism may not contribute directly to the development of tardive dyskinesia in schizophrenia, but it may be involved in the excited behavior of tardive dyskinesia patients PMID: 25336319
C-1021T polymorphism not associated with orthostatic hypotensive risk in a Chinese population PMID: 24990418
this study shows the influence of 444 G/A (rs 1108580) and -1021 C/T (rs 1611115) polymorphisms of the dopamine beta-hydroxylase (DBH) gene on clinical parameters of the trajectory of alcohol dependence. PMID: 26356164
Identification of DBH variants with strong effects makes it possible to take advantage of Mendelian randomization approaches to test causal effects of this intermediate trait on disease. PMID: 24986918
DBH5'-Ins/Del and -1021C/T polymorphism in promoter region of DBH gene was associated with major depressive disorder. PMID: 24888991
The genetic polymorphism in dopamine beta-hydroxylase is associated with methamphetamine-induced paranoia and influences smoking initiation. PMID: 24521142
This study demonstrated the importance of DBH -1021C/T as a genetic basis of empathy and in predicting individual differences in social and affective processing. PMID: 23988761
Demonstrate profound effects of DBH variants on expression in 2 sympathetically innervated organs, liver and lung, and association of these variants with clinical phenotypes responsive to peripheral sympathetic tone. PMID: 25326128
An association between dopamine-beta hydroxylase (DBH) promoter polymorphisms (a 5'-ins/del and a GTn repeats) and a history of suicide attempt in 223 chronic schizophrenia individuals, was studied. PMID: 24710129
This study demonistrated that the Polymorphisms of dopamine beta hydroxylase is not not associated with Alzheimer's disease. PMID: 24201835
Individuals with the normal DbetaH activity genotype (CC) showed no differential response to levodopa treatment for cocaine dependence PMID: 24809448
role for DBH promoter variation in long-term renal decline rate PMID: 24391727
Data suggest that the interaction of single nucleotide polymorphism (SNP) of interleukin-10 (IL-10) and and dopamine beta-hydroxylase (DBH) may play a role in the general symptoms on PANSS in schizophrenic patients with Tardive dyskinesia (TD). PMID: 23951054
This study show DBH5'-ins/del heterozygotes (associated with average level of plasma DbetaH activity) showed increased post-error slowing compared to del/del homozygotes and ins/ins homozygotes (associated with low and high level of plasma DbetaH activity. PMID: 23962674
This study presents evidence for a potentially functional DBH variant influencing the risk for alcohol dependence while other comorbid conditions are not independently influenced PMID: 23906995
These results suggested that dopamine beta hydroxylase gene (DBH) might be related to attention-deficit hyperactivity disorder (ADHD)symptoms in korean children. PMID: 23692268
This study identified that the DBH5'-Ins/Del polymorphism may contribute to the susceptibility to first-episode schizophrenic patients. PMID: 23707643
genetic association studies in the Netherlands: Data suggest that an SNP in DBH (rs2519152) is associated with attention deficit disorder with hyperactivity (but not substance abuse disorders). [PILOT STUDY] PMID: 22841130
although the DBH 5'-Ins/Del polymorphism was not associated with susceptibility to TD in patients with schizophrenia, it might be related to positive symptoms of schizophrenia PMID: 23559427
This study showed genotypes-independent decrease in pDBH activity in patients with AD compared to enzyme activity in age-matched healthy controls. We did not confirm the association of low activity T allele variant of the DBH C-970T and alzheimer disease. PMID: 23416088
the dopamine beta-hydroxylase (DBH) polymorphism (DBH -1021C/T) that is associated with low DbetaH levels also was associated with greater improvement in cocaine positive urines in the vaccine-treated group. PMID: 23458673
-1021TT genotype may be implicated with a more progressive nature of heroin addiction, although DBH -1021C/T is unlikely to be involved in the risk of heroin addiction. PMID: 23510745
DBH rs7239728 imparted significant migraine risk at genotypic, allelic & carrier analyses. 2 genotype interactions between ANKK1 rs1800497 & DBH rs72393728 polymorphisms showed significant risks. PMID: 22875483
DBH and MAOA can influence human attentional biases, and there is a gene-gene interaction between the DBH and MAOA on attentional bias for negative expressions. PMID: 23054588
the DBH genotype of a patient could be used to identify a subset of individuals for which disulfiram treatment might be an effective pharmacotherapy for cocaine dependence. PMID: 22906516
The 1603C>T polymorphism of the DBH gene is associated with susceptibility to bipolar disorder in a Turkish population. PMID: 23384717
The distribution of AA genotype and A allele frequencies of rs5320 in the dopamine beta-hydroxylase gene in ADHD children differed significantly from that in healthy controls. PMID: 22895683
These results suggest that the DBH 5'-Ins/Del polymorphism may influence smoking severity among schizophrenic smokers. PMID: 22871345

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