Recombinant Human DKK1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1618
Recombinant Human DKK1 Protein (His Tag)

Recombinant Human DKK1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1618
Catalog No.: BLPSN-1618

Product Overview

Tag His
Host Species Human
Accession NP_036374.1
Synonym DKK-1, SK
Background Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.
Description A DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) was expressed with a C-terminal His tag.
Source HEK293
Predicted N Terminal Thr 32 or Ser 35
AA Sequence Met 2-His 266
Molecular Weight The recombinant human DKK1 protein consists of 235 a.a. and has a calculated molecular mass of 25.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh DKK1 is approximately 45 kDa due to glycosylation.
Purity >90% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 ug/ml in the presence of 10 ng/mL of mouse Wnt3a.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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