Recombinant Human DKK1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1618
Recombinant Human DKK1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1618
Catalog No.: BLPSN-1618
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_036374.1 |
| Synonym | DKK-1, SK |
| Background | Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers. |
| Description | A DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) was expressed with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Thr 32 or Ser 35 |
| AA Sequence | Met 2-His 266 |
| Molecular Weight | The recombinant human DKK1 protein consists of 235 a.a. and has a calculated molecular mass of 25.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh DKK1 is approximately 45 kDa due to glycosylation. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 ug/ml in the presence of 10 ng/mL of mouse Wnt3a. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
