Recombinant Human Cyclin E Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1519
Recombinant Human Cyclin E Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1519
Catalog No.: BLPSN-1519
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | NP_001229.1 |
| Synonym | CCNE |
| Background | Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells. |
| Description | A DNA sequence encoding the full length of human CCNE1 isoform 1 (NP_001229.1) (Met 1-Ala 410) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met 1-Ala 410 |
| Molecular Weight | The recombinant human CCNE1/GST chimera consists of 647 a.a. and has a calculated molecular mass of 75 KDa. It migrates as an approximately 70 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >82% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human HG3C-CCNE1 at 10 ug/ml (100 ul/well) can bind biotinylated human GST-CDK4, The EC50 of biotinylated human GST-CDK4 is 45.0-106.0 ng/ml. |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, 1mM GSH, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
