Recombinant Human CTHRC1 Protein (His Tag)

Name: Recombinant Human CTHRC1 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1468
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	NP_612464.1
Background	Collagen triple helix repeat-containing protein 1, also known as Protein NMTC1, and CTHRC1, is a secreted protein that is glycosylated and highly conserved from lower chordates to mammals. CTHRC1 expression was not detectable in normal arteries. However, it is transiently expressed in the arterial wall in response to injury where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration. A short collagen motif with 12 Gly-X-Y repeats appears to be responsible for trimerization of the CTHRC1 protein and this renders the molecule susceptible to cleavage by collagenase. CTHRC1 overexpression caused a dramatic reduction in collagen type I mRNA and protein levels. Currently available data indicate that Cthrc1 expression in vascular cells regulates transforming growth factor beta responsiveness, thereby impacting transforming growth factor beta target genes, including collagens. Additionally, CTHRC1 increases bone mass as a positive regulator of osteoblastic bone formation and offers an anabolic approach for the treatment of osteoporosis.
Description	A DNA sequence encoding the human CTHRC1 (NP_612464.1) (Met 1-Lys 243) was fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Ser 31
AA Sequence	Met 1-Lys 243
Molecular Weight	The recombinant human CTHRC1 consists of 224 a.a. and has a predicted molecular mass of 24.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CTHRC1 is approximately 28-33 kDa due to glycosylation.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	May act as a negative regulator of collagen matrix deposition.
Subcellular Location	Secreted, extracellular space, extracellular matrix.
Database References	HGNC: 18831 OMIM: 610635 KEGG: hsa:115908 STRING: 9606.ENSP00000330523 UniGene: Hs.405614
Associated Diseases	Barrett esophagus (BE)
Tissue Specificity	Isoform 1 is expressed in calcified atherosclerotic plaque and chondrocyte-like cells.

Gene Functions References

CTHRC1 serves as a pro-metastatic gene that contributes to NSCLC invasion and metastasis, which are mediated by upregulated MMP7 and MMP9 expression. Targeting CTHRC1 may be beneficial for inhibiting NSCLC metastasis. PMID: 29631554
High Collagen Triple Helix Repeat Containing 1 Expression is associated with Osteosarcoma. PMID: 29970438
E6/E7-p53-POU2F1-CTHRC1 axis promotes cervical cancer cell invasion and metastasis PMID: 28303973
IL-1beta and CTHRC1 are upregulated in patients with Osteoarthritis. PMID: 29393342
CTHRC1 interacts with integrin beta3 and accelerates the FAK phosphorylation to promote ovarian cancer cell adhesion, migration and invasion in vitro and in vivo. PMID: 29021002
CTHRC1 plays a pivotal role in a great many fields, including increases bone mass, prevents myelination, reverses collagen synthesis in keloid fibroblasts, and increases fibroblast-like synoviocytes migration speed and abundant production of arthritic pannus in rheumatoid arthritis PMID: 28901303
CTHRC1, negatively regulated by miR-30c, promoted cell proliferation, invasion and migration and suppressed cell apoptosis in breast cancer, which might be by activating GSK-3beta/beta-catenin signaling and inhibiting Bax/Caspase-9/Caspase-3 signaling respectively. PMID: 28697793
Our data suggest that CTHRC1 may act as an oncogenic driver in progression and metastasis of ESCC, and may serve as a potential biomarker for prognosis and personalized therapy. PMID: 28645305
The negative and sensitivity-predictive values of CTHRC1 staining were excellent for both lymph node and peritoneal metastases. PMID: 27870703
High CTHRC1 expression is associated with metastatic melanomas. PMID: 26918341
CTHRC1 was established as a novel marker of activated synoviocytes in murine experimental arthritis and rheumatoid arthritis PMID: 27430622
ANOS1 and its co-expression partner, CTHRC1, promote the development and metastasis of colorectal cancer. PMID: 28854193
Expression of CTHRC1 was significantly higher in Wilms' tumor compared to the expression in the adjacent non-cancerous tissues. High tumor expression of CTHRC1 was associated with tumor size, clinical stage, histopathological type, and vascular invasion/metastasis. Patients with high CTHRC1 expression also exhibited a shorter survival. PMID: 27230801
CTHRC1 expression is significantly upregulated in human masticatory mucosa during wound healing. PMID: 28005267
CTHRC1 downregulation inhibited proliferation. PMID: 28281968
The knockdown of CTHRC1 exerts inhibitory effects on the proliferation and migration ability of glioblastoma cells. PMID: 28277194
CTHRC1 may play a role in the progression of ovarian cancer. PMID: 27779718
this study shows that the serum CTHRC1 level was significantly higher in the influenza A virus infection patients than in the healthy individuals; the influenza A virus non-structural protein NS1 upregulates the expression of CTHRC1 protein PMID: 27718266
Gene expression levels of three randomly selected DEGs, VCAN, COL5A1 and KCNJ16, were examined using RT-PCR in 10 ATC samples.. angiogenesis was activated by the high expression of CTHRC1, VCAN and POSTN, providing necessary nutrition for tumor cells PMID: 27599582
increased CTHRC1 expression is associated with advanced TNM stage, increased LN metastasis and tumor size, and decreased OS and DFS, indicating that CTHRC1 may be a biomarker for prognosis of cancer patients PMID: 27323076
High CTHRC1 expression is associated with Osteosarcoma. PMID: 27043295
Authors showed that ectopic transfection of CTHRC1 in EOC cells up-regulated the expression of EMT markers such as N-cadherin and vimentin, and EMT-associated transcriptional factor Snail. PMID: 26452130
CTHRC1 is secreted both by colorectal epithelia cells and stromal fibroblasts. CTHRC1 overexpression promotes colorectal cancer cell migration, invasion and proliferation in vitro. PMID: 26722469
determined the mRNA and protein expression of CTHRC1 in oral squamous cell carcinoma and evaluated the clinical and prognostic impact of CTHRC1 overexpression PMID: 26664254
CTHRC1 has a novel role in viral infection. PMID: 26180054
HBV facilitates HCC development through activating the oncoprotein CTHRC1. PMID: 25263696
CTHRC1 was overexpressed in human non-small cell lung cancer tissues and non-small cell lung cancer cell lines at the protein and mRNA level. PMID: 25238260
let-7b may directly target Cthrc1 and function as a tumor suppressor gene in gastric cancer. PMID: 25510669
CTHRC1 acts as a prognostic factor and promotes invasiveness of gastrointestinal stromal tumors by activating Wnt/PCP-Rho signaling. PMID: 24726140
Cthrc1 overexpression was associated with non-small cell lung cancers. PMID: 25139095
Cthrc1 is a pituitary hormone with significantly elevated levels in subjects carrying variant alleles of the melanocortin-1 receptor as wells as in patients with inflammatory conditions. PMID: 24945147
CTHRC1 has the potential to be a new biomarker for the aggressive hepatocellular carcinoma PMID: 24841500
CTHRC1 expression is elevated in human colon cancer cell lines and clinical specimens, and promotes cancer cell invasivity through ERK-dependent induction of MMP9 expression. PMID: 24504172
Studied CTHRC1 expression in human breast cancer tissue. A significant increase of CTHRC1 mRNA expression was seen in breast cancer tissue compared to the normal tissue from the same patients using RT-PCR and real-time PCR. PMID: 23658133
Overexpression of CTHRC1 in hepatocellular carcinoma promotes tumor invasion and predicts poor prognosis. PMID: 23922981
Rs35500845 in the CTHRC1 gene was associated with Paget's disease of bone in the French-Canadian population. PMID: 24370779
high Cthrc1 expression was an independent prognostic factor for both overall survival and disease-free survival of patients with gastric carcinoma PMID: 24746208
Data suggest that in oral squamous cell carcinoma (OSCC), dysregulation of canonical Wnt signaling and DPAGT1-dependent N-glycosylation induces CTHRC1, thereby driving OSCC cell migration and tumor spread. PMID: 23703614
this is the first demonstration of Cthrc1 as a marker of the severity of the disease progression in the dystrophic muscles. PMID: 23274062
the results of our study suggest that increased expression of CTHRC1 is associated with peritoneal carcinomatosis in Colorectal cancer patients PMID: 23359115
CTHRC1 has a role in pancreatic cancer progression and metastasis by regulating migration and adhesion activities of cancer cells. PMID: 23222813
Data indicate that the upregulated expression of collagen triple helix repeat containing 1 (CTHRC1) in gastric carcinogenesis contributes to tumor cell invasion and metastasis. PMID: 22590977
Three major genes, MSR1, ASCC1, and CTHRC1 were associated with Barrett esophagus/esophageal adenocarcinoma PMID: 21791690
CTHRC1 is transiently expressed in the arterial wall in response to injury where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration. PMID: 15618538
Aberrant expression of CTHRC1 is widely present in human solid cancers and seems to be associated with cancer tissue invasion and metastasis. PMID: 16778098
Intracellular localization of Cthrc1 characterizes differentiated smooth muscle. PMID: 18467647

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