Name: Recombinant Human CST6 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1463
Availability: InStock

Recombinant Human CST6 Protein (His Tag)

Catalog No.: BLPSN-1463

Product Overview

Tag	His
Host Species	Human
Accession	NP_001314.1
Background	Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene.
Description	A DNA sequence encoding the human cystatin E/M protein (NP_001314.1) (Met 1-Met 149) was expressed with a C-terminal His-tag.
Source	HEK293
Predicted N Terminal	Arg 29
AA Sequence	Met 1-Met 149
Molecular Weight	The mature form of recombinant human cystatin E/M consists of 132 a.a. and predicts a molecular mass of 15 kDa. It exists as two major forms with an identical N-terminal sequence. As a result of glycosylation, rh cystatin E/M migrates as doublets with the molecular mass of approximately 15 and 21 kDa respectively in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 25mM MES, 0.15M NaCl, pH 6.5.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.