Recombinant Human CSNK1G1 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1460
Recombinant Human CSNK1G1 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1460
Catalog No.: BLPSN-1460
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | NP_071331.2 |
| Synonym | CK1gamma1 |
| Background | Casein kinase I isoform gamma-1, also known as CSNK1G1, is a member of the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Thecasein kinase I family of protein kinases are serine / threonine-selective enzymes that function as regulators ofsignal transductionpathways in most eukaryotic cell types. Casein has been used as a substrate since the earliest days of research on protein phosphorylation. Casein kinase activity associated with the endoplasmic reticulum of mammary glands was first characterized in 1974 and its activity was shown to not depend on cyclic AMP. The CKI family of monomeric serine-threonine protein kinases is found in eukaryotic organisms from yeast to human. Mammals have seven family members: alpha, beta 1, gamma 1, gamma 2, gamma 3, delta, and epsilon. The family members have the highest homology in their kinase domains (53%-98% identical) and differ from most other protein kinases by the presence of the sequence S-I-N instead of A-P-E in kinase domain VIII. The CKI family members appear to have similar substrate specificity and substrate selection is thought to be regulated via subcellular localization and docking sites in specific substrates. |
| Description | A DNA sequence encoding full length of human CSNK1G1 (NP_071331.2) (Met 1-Lys 422) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met 1-Lys 422 |
| Molecular Weight | The recombinant human CSNK1G1/GST chimera consists of 659 a.a. and has a calculated molecular mass of 76.3 kDa. It migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >94% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | The specific activity was determined to be 4 nmol/min/mg using casein as substrate. |
| Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
