Name: Recombinant Human Cripto Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1430
Availability: InStock

Recombinant Human Cripto Protein (His Tag)

Catalog No.: BLPSN-1430

Product Overview

Tag	His
Host Species	Human
Accession	AAH22393.1
Synonym	CR, CRGF, CRIPTO
Background	Cripto/TDGF1 is a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family. EGF-CFC family member proteins share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. Before gastrulation, Cripto is asymmetrically expressed in a proximal-distal gradient in the epiblast, and subsequently is expressed in the primitive streak and newly formed embryonic mesoderm. These proteins play key roles in intercellular signaling pathways during vertebrate embryogenesis. Mutations in Cripto/TDGF1 can cause autosomal visceral heterotaxy. Cripto/TDGF1 is involved in left-right asymmetric morphogenesis during organ development. Cripto signalling is essential for the conversion of a proximal-distal asymmetry into an orthogonal anterior-posterior axis. The mechanism of inhibitory effects of the Cripto includes both cancer cell apoptosis, activation of c-Jun-NH(2)-terminal kinase and p38 kinase signaling pathways and blocking of Akt phosphorylation. Thus, Cripto is a unique target, and Immunohistochemistry to Cripto could be of therapeutic value for human cancers.
Description	A DNA sequence encoding the human TDGF1 (AAH22393.1) (Met 1-Thr 172) with a C-terminal His tag was expressed.
Source	HEK293
Predicted N Terminal	Leu 31
AA Sequence	Met 1-Thr 172
Molecular Weight	The secreted recombinant human TDGF1 comprises 153 a.a. with a predicted molecular mass of 17.4 kDa. As a result of glycosylation, the apparent molecular mass of rhTDGF1 is approximately 25-30 kDa in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized human TDGF1 at 2 ug/ml (100 ul/well) can bind human ALK4 with a linear range of 0.0068-0.16 ug/ml.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.