Recombinant Human Creatine kinase B Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1418
Recombinant Human Creatine kinase B Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1418
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P12277 |
| Synonym | B-CK, BCK, CKBB, HEL-211, HEL-S-29 |
| Background | CKB(Creatine kinase B type) contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. It belongs to the ATP:guanido phosphotransferase family. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CKB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. CKB reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer in brain, smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone etc. |
| Description | A DNA sequence encoding the human CKB (P12277)(Pro2-Lys381) was fused with a His tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | His |
| AA Sequence | Pro2-Lys381 |
| Molecular Weight | The recombinant human CKB consists of 398 a.a. and has a calculated molecular mass of 44.8 kDa. The recombinant protein migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% gly, pH 8.0.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
