Recombinant Human CPLX3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1407
Recombinant Human CPLX3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1407
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | Q8WVH0 |
Synonym | CPX-III, CPXIII, Nbla11589 |
Background | CPLX3, also known as complexin 3, belongs to the complexin/synaphin family. As a SNARE-binding protein, complexin (CPX), can act either as a facilitator or as an inhibitor of membrane fusion, constituting a controversial dilemma. CPX acts sequentially on assembling SNAREpins, first facilitating zippering by nearly doubling the distance at which v- and t-SNAREs can engage and then clamping them into a half-zippered fusion-incompetent state. Specifically, the central helix of CPX allows SNAREs to form this intermediate energetic state at 9-15 nm but not when the bilayers are closer than 9 nm. Stabilizing the activated-clamped state at separations of less than 9 nm requires the accessory helix of CPX, which prevents membrane-proximal assembly of SNAREpins. CPLX3 binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A. |
Description | A DNA sequence encoding the human CPLX3 (Q8WVH0) (Met1-Lys154) was expressed with an N-terminal His tag. |
Source | HEK293 |
Predicted N Terminal | His |
AA Sequence | Met1-Lys154 |
Molecular Weight | The recombinant human CPLX3 comprises 174 a.a. and has a predicted molecular mass of 19.5 kDa. The apparent molecular mass of the protein is approximately 29-32 kDa in SDS-PAGE under reducing conditions. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |