Recombinant Human Contactin 3 Protein (708 Asp/Asn, His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1393
Recombinant Human Contactin 3 Protein (708 Asp/Asn, His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1393
Catalog No.: BLPSN-1393
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_065923.1 |
| Synonym | BIG-1, PANG, PCS |
| Background | Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 198s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-3, also known as CNTN3 ( BIG-1 in rat and PANG in mouse ), is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. In the brain, Contactin-3 is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth. |
| Description | A DNA sequence encoding the human CNTN3 (NP_065923.1) (Met 1-Ser1002, 708 Asp/Asn) precursor was expressed with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Glu 20 |
| AA Sequence | Met 1-Ser1002, 708 Asp/Asn |
| Molecular Weight | The recombinant human CNTN3 consists of 994 a.a. and has a calculated molecular mass of 109.2 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rh CNTN3 is approximately 120-130 kDa due to glycosylation. |
| Purity | >97% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CNTN3 coated plates (0.8 ug/ml and 100 ul/well), approximately 35%-55% will adhere specifically after 60 minutes at 37°C. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
