Recombinant Human Cofilin Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1372
Recombinant Human Cofilin Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1372
Catalog No.: BLPSN-1372
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P23528 |
| Synonym | CFL, HEL-S-15 |
| Background | CFL1, also known as n-cofilin, is a member of the ADF/Cofilin family. This family comprises three genes: CFL1, CFL2 and DSTN (destrin). ADF/Cofilin family members bind G-actin monomers and depolymerize actin filaments through two mechanisms: severing and increasing the off-rate for actin monomers from the pointed end. Cofilin also binds with other proteins such as myosin, tropomyosin, alpha-actinin, gelsolin and scruin. These proteins compete with cofilin for actin binding. Сofilin also plays a role in innate immune response. CFL1 contains 1 ADF-H domain and is widely distributed in various tissues. It is important for normal progress through mitosis and normal cytokinesis. |
| Description | A DNA sequence encoding the human CFL1 (P23528) (Met1-Leu166) was expressed with a His tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | His |
| AA Sequence | Met1-Leu166 |
| Molecular Weight | The recombinant human CFL1 consists of 181 a.a. and predicts a molecular mass of 20.3 KDa. It migrates as an approximately 21 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
