Recombinant Human COCH Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1369
Recombinant Human COCH Protein (His Tag)

Recombinant Human COCH Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1369
Catalog No.: BLPSN-1369

Product Overview

Tag His
Host Species Human
Accession NP_001128530.1
Synonym COCH-5B2, COCH5B2, DFNA9
Background Cochlin, also known as COCH-5B2 and COCH, is a secreted protein which contains one LCCL domain and two VWFA domains. It is an abundant inner ear protein expressed as multiple isoforms. Its function is also unknown, but it is suspected to be an extracellular matrix component. Cochlin and type II collagen are major constituents of the inner ear extracellular matrix, and Cochlin constitutes 7% of non-collagenous protein in the inner ear, the cochlin isoforms can be classified into three subgroups, p63s, p44s and p4s. The expression of cochlin is highly specific to the inner ear. Eleven missense mutation and one in-frame deletion have been reported in the COCH gene, causing hereditary progressive sensorineural hearing loss and vestibular dysfunction, deafness autosomal dominant type 9 (DFNA9). The co-localization of cochlin and type II collagen in the fibrillar substance in the subepithelial area indicate that cochlin may play a role in the structural homeostasis of the vestibule acting in concert with the fibrillar type II collagen bundles. Defects in COCH may contribute to Meniere disease which is an autosomal dominant disorder characterized by hearing loss associated with episodic vertigo.
Description A DNA sequence encoding the mature form of human COCH (NP_001128530.1) (Glu 25-Gln 550) was expressed, with a His tag at the N-terminus.
Source HEK293
Predicted N Terminal His
AA Sequence Glu 25-Gln 550
Molecular Weight The recombinant full length of human COCH consists of 542 a.a. and has a calculated molecular mass of 59.4 kDa. In SDS-PAGE under reducing conditions, rhCOCH migrates as three bands with apparent molecular mass of 66, 48 and 18 kDa, corresponding to the alternative splicing isoforms.
Purity >92% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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