Recombinant Human CMBL Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1333
Recombinant Human CMBL Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1333
Catalog No.: BLPSN-1333
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | Q96DG6 |
| Synonym | JS-1 |
| Background | Carboxymethylenebutenolidase (CMBL), also known as 4-carboxymethylenebut-2-en-4-olide lactonohydrolase, maleylacetate enol- lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase, is a hydrolase specially belonging to the family of hydrolases. It maily acts on carboxylic ester bonds. CMBL is a human homolog of Pseudomonas dienelactone hydrolase involved in the bacterial halocatechol degradation pathway. The ubiquitous expression of human CMBL gene transcript in various tissues was observed. CMBL was demonstrated to be the primary olmesartan medoxomil (OM) bioactivating enzyme in the liver and intestine. The recombinant human CMBL expressed in mammalian cells was clearly shown to activate OM. The recombinant CMBL also converted other prodrugs having the same ester structure as OM, faropenem medoxomil and lenampicillin, to their active metabolites. CMBL exhibited a unique sensitivity to chemical inhibitors, thus, being distinguishable from other known esterases. |
| Description | A DNA sequence encoding the human CMBL (Q96DG6) (Met 1-Met 245) was expressed, with a N-terminal His tag. |
| Source | E.coli |
| Predicted N Terminal | Met |
| AA Sequence | Met 1-Met 245 |
| Molecular Weight | The recombinant human CMBL comprises 260 a.a. and has a calculated molecular mass of 30 kDa. It migrates as an approximately 28 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >97% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20mM Tris, 0.1% Brij35, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
