Recombinant Human CLEC-2 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1301
Recombinant Human CLEC-2 Protein (His Tag)

Recombinant Human CLEC-2 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1301
Catalog No.: BLPSN-1301

Product Overview

Tag His
Host Species Human
Accession NP_057593.3
Synonym 1810061I13Rik, CLEC2, CLEC2B, PRO1384, QDED721
Background CLEC1B, also known as CLEC2, is a C-type lectin-like receptor expressed in myeloid cells and NK cells. Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 and NKG2D, that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. CLEC2 contains 1 C-type lectin domain and is expressed preferentially in the liver. It acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets.
Description A DNA sequence encoding the human CLEC1B (NP_057593.3) extracellular domain (Gln 58-Pro 229) with a N-terminal His tag was expressed.
Source HEK293
Predicted N Terminal His
AA Sequence Gln 58-Pro 229
Molecular Weight The recombinant human CLEC1B comprises 188 a.a. with a predicted molecular mass of 22.7 kDa. As a result of glycosylation, the apparent molecular mass of rh CLEC1B is approximately 35-38 kDa in SDS-PAGE under reducing conditions.
Purity >76% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human Podoplanin at 10 ug/mL (100 ul/well) can bind-  biotinylated human CLEC1B-His, The EC50 of biotinylated human CLEC1B-His is 0.71 ug/mL.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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