Recombinant Human CHI3L2 Protein (His Tag)

Name: Recombinant Human CHI3L2 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1243
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	NP_003991.2
Synonym	CHIL2, YKL-39, YKL39
Background	Chondrocyte protein 39 (YKL-39), also known as Chitinase 3-like 2 (CHI3L2), is a secretory protein of articular chondrocytes belonging to the glycosyl hydrolase 18 family. It highest expression is in chondrocytes, followed by synoviocytes, lung and heart. YKL-39/CHI3L2 is not detected in spleen, pancreas, and liver. YKL-39/CHI3L2 may also be expressed in developing brain and placenta. YKL-39/CHI3L2, a cartilage-related protein, is found to induce arthritis accompanied by pathologic changes in bone and cartilage. A better understanding of the immune response against cartilage-related components including YKL-39 may help to elucidate the pathological processes of arthritic disorders. Up regulation of YKL-39/CHI3L2 in osteoarthritic cartilage suggests that YKL-39/CHI3L2 may be a more accurate marker of chondrocyte activation than YKL-4, although it has yet to be established as a suitable marker in synovial fluid and serum. The decreased expression of YKL-4 by osteoarthritic chondrocytes is surprising as increased levels have been reported in rheumatoid and osteoarthritic synovial fluid, where it may derive from activated synovial cells or osteophytic tissue or by increased matrix destruction in the osteoarthritic joint. YKL-39 and YKL-4 are potentially interesting marker molecules for arthritic joint disease because they are abundantly expressed by both normal and osteoarthritic chondrocytes.
Description	A DNA sequence encoding the human CHI3L2 (NP_003991.2) extracellular domain (Met 1-Leu 390) was fused with a His tag at the C-terminus and a signal peptide at the N-terminus.
Source	HEK293
Predicted N Terminal	Lys 28
AA Sequence	Met 1-Leu 390
Molecular Weight	The recombinant human CHI3L2 consists of 374 a.a. and has a predicted molecular mass of 42.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCHI3L2 is approximately 43 kDa.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.
Subcellular Location	Secreted.
Protein Families	Glycosyl hydrolase 18 family
Database References	HGNC: 1933 OMIM: 601526 KEGG: hsa:1117 STRING: 9606.ENSP00000358763 UniGene: Hs.514840
Tissue Specificity	Highest expression in chondrocytes, followed by synoviocytes, lung and heart. Not detected in spleen, pancreas, and liver. May also be expressed in developing brain and placenta.

Gene Functions References

This study shown that CHI3L2 is regulated differently in healthy and late onset Alzheimer"s disease brain depending on the sex. PMID: 27653898
Structural analysis demonstrates that YKL-39 interacts with chitooligosaccharides through hydrogen bonds and hydrophobic interactions. Thermodynamic analysis indicates that binding of chitooligosaccharide to YKL-39 is mainly driven by enthalpy. PMID: 25477513
These data suggest that hYKL-39 is a novel growth and differentiation factor involved in cartilage homeostasis. PMID: 23291184
YKL-39 possesses a chitinase-like fold, but lacks key active-site residues required for catalysis. PMID: 22742450
ERK1/2 phosphorylation by CHI3L2 inhibits cell mitogenesis and proliferation. PMID: 22211103

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.