Recombinant Human CEACAM6 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1214
Recombinant Human CEACAM6 Protein (His Tag)

Recombinant Human CEACAM6 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1214
Catalog No.: BLPSN-1214

Product Overview

Tag His
Host Species Human
Accession NP_002474.3
Synonym CD66c, CEA, CEAL, NCA
Background Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), also known as nonspecific crossreacting antigen (NCA) and CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily. It s a glycosylphosphatidylinositol-linked immunoglobulin superfamily member that is overexpressed in a variety of human cancers, including colon, breast and lung and is associated with tumourigenesis, tumour cell adhesion, invasion and metastasis. CEACAM6 is a unique mediator of migration and invasion of drug resistant oestrogen-deprived breast cancer cells, and this protein could be an important biomarker of metastasis. CEACAM6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.
Description A DNA sequence encoding the human CEACAM6 (NP_002474.3) (Met 1-Gly 320) with a C-terminal His tag was expressed.
Source HEK293
Predicted N Terminal Lys 35
AA Sequence Met 1-Gly 320
Molecular Weight The secreted recombinant human CEACAM6 comprises 297 a.a. with a predicted molecular mass of 32.6 kDa. As a result of glycosylation, rh CEACAM6 migrates as an approximately 45-55 kDa band in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1. Measured by its binding ability in a functional ELISA.2. Immobilized human CEACAM6-his at 10 ug/mL (100 ul/well) can bind biotinylated human CEACAM8-his, The EC50 of biotinylated human CEACAM8-his is 0.17 ug/mL.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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