Recombinant Human Caspase-14 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0589
Recombinant Human Caspase-14 Protein (His Tag)

Recombinant Human Caspase-14 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0589
Catalog No.: BLPSN-0589

Product Overview

Tag His
Host Species Human
Accession NP_036246.1
Background Caspase 14 is a member of the caspase family. Caspases are a kind of cysteine proteinase consisting of a prodomain plus large and small catalytic subunits, that play a central role in cell apoptosis. Caspase 14 possesses an unusually short prodomain and is highly expressed in embryonic tissues but absent from most of the adult tissues except for the skin, which suggests a role in ontogenesis and skin physiology. Unlike the other short prodomain caspases(caspase-3, caspase-6, and caspase-7), Caspase 14 was not processed by multiple death stimuli including activation of members of the tumor necrosis factor receptor family and expression of proapaptotic members of the bcl-2 family. Caspase 14 has been described to be processed and activated by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may take part in keratinocyte terminal differentiation, which is essential for the skin barrier.
Description A DNA sequence encoding the human CASP14 (NP_036246.1) (Ser 2-Gln 242) was expressed, with a His tag at the N-terminus.
Source E.coli
Predicted N Terminal Met
AA Sequence Ser 2-Gln 242
Molecular Weight The recombinant human CASP14 consisting of 248 a.a. and has a calculated molecular mass of 28.5 kDa. The apparent molecular mass of the protein is approximately 30 kDa in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin Please contact us for more information.
Bioactivity Measured by its ability to bind biotinylated Cynomolgus IL18 in a functional ELISA.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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