Recombinant Human CARKL Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0582
Recombinant Human CARKL Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0582
Catalog No.: BLPSN-0582
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | NP_037408.2 |
| Synonym | CARKL, SHK |
| Background | CARKL, also known as SHPK, is a nonprotein kinase of glucose metabolism. CARKL has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. CARKL catalyzes an orphan reaction in the pentose phosphate pathway, refocusing cellular metabolism to a high-redox state upon physiological or artificial downregulation. CARKL-dependent metabolic reprogramming is required for proper M1- and M2-like macrophage polarization and uncover a rate-limiting requirement for appropriate glucose flux in macrophage polarization. |
| Description | A DNA sequence encoding the human SHPK (NP_037408.2) (Ala2-Ser478) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Ala2-Ser478 |
| Molecular Weight | The recombinant human SHPK/GST chimera consists of 714 a.a. and has a calculated molecular mass of 79.2 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
