Recombinant Human CARHSP1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0580
Recombinant Human CARHSP1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0580
Catalog No.: BLPSN-0580
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | Q9Y2V2 |
| Synonym | CRHSP-24, CSDC1 |
| Background | CARHSP1 is a biomarker for diabetic complications. Adenovirus-mediated CARHSP1 overexpression and siRNA-mediated knockdown experiments were performed to characterize the role of CARHSP1 in the regulation of gluconeogenic gene expression. CARHSP1 is regulated by nutrient status in the liver and functions at the transcriptional level to negatively regulate gluconeogenic genes, including the glucose-6-phosphatase catalytic subunit (G6Pc) and phosphoenolpyruvate carboxykinase 1 (PEPCK1). In addition, it is found that CARHSP1 can physically interact with peroxisome proliferator-activated receptor-alpha (PPARalpha) and inhibit its transcriptional activity. Both pharmacological and genetic ablations of PPARalpha attenuate the inhibitory effect of CARHSP1 on gluconeogenic gene expression in hepatocytes. |
| Description | A DNA sequence encoding the human CARHSP1(Q9Y2V2 ) (Met1-Ser147) was expressed with a His tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | His |
| AA Sequence | Met1-Ser147 |
| Molecular Weight | The recombinant human CARHSP1 consists of 162 a.a. and predicts a molecular mass of 17.7 KDa. It migrates as an approximately 21 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 50mM Tirs, 10% Glycerol, 200mM NaCl, pH 8.0.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
