Recombinant Human Carboxypeptidase B1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0569
Recombinant Human Carboxypeptidase B1 Protein (His Tag)

Recombinant Human Carboxypeptidase B1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0569
Catalog No.: BLPSN-0569

Product Overview

Tag His
Host Species Human
Accession NP_001862.2
Synonym CPB, PASP, PCPB
Background Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Description A DNA sequence encoding the human CPB1 (NP_001862.2) (Met 1-Tyr 417) was expressed with a C-terminal His tag.
Source HEK293
Predicted N Terminal His 16
AA Sequence Met 1-Tyr 417
Molecular Weight The recombinant human CPB1 consists of 413 a.a. and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 45 kDa band.
Purity >98% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg, as measured using the wavelength at 254 nm.The specific activity is >10000 pmoles/min/ug.
Formulation Lyophilized from sterile 25mM MES, 0.1 M NaCl, pH 6.5.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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