Recombinant Human Carboxypeptidase B1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0569

Recombinant Human Carboxypeptidase B1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0569
Catalog No.: BLPSN-0569
Product Overview
Tag | His |
Host Species | Human |
Accession | NP_001862.2 |
Synonym | CPB, PASP, PCPB |
Background | Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin. |
Description | A DNA sequence encoding the human CPB1 (NP_001862.2) (Met 1-Tyr 417) was expressed with a C-terminal His tag. |
Source | HEK293 |
Predicted N Terminal | His 16 |
AA Sequence | Met 1-Tyr 417 |
Molecular Weight | The recombinant human CPB1 consists of 413 a.a. and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 45 kDa band. |
Purity | >98% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg, as measured using the wavelength at 254 nm.The specific activity is >10000 pmoles/min/ug. |
Formulation | Lyophilized from sterile 25mM MES, 0.1 M NaCl, pH 6.5. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |