Name: Recombinant Human Carboxypeptidase A2 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0566
Availability: InStock

Recombinant Human Carboxypeptidase A2 Protein (His Tag)

Catalog No.: BLPSN-0566

Product Overview

Tag	His
Host Species	Human
Accession	NP_001859.1
Synonym	CPA2
Background	Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
Description	A DNA sequence encoding the pre pro form of human CPA2 (NP_001859.1) (Met 1-Tyr 417) was expressed with a C-terminal His tag.
Source	HEK293
Predicted N Terminal	Leu 17
AA Sequence	Met 1-Tyr 417
Molecular Weight	The secreted recombinant human CPA2 existing as the proform consists of 412 a.a. and has a predicted molecular mass of 46 kDa also as estimated by SDS-PAGE under reducing conditions.
Purity	>90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to cleave a colorimetric peptide substrate, N-acetyl-Phe-Thiaphe-OH (N-Ac-PSP, Peptide International's Catalog# STP-3621-PI), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405 nm and the extinction coefficient of 13,260 M-1 cm-1. The specific activity is >4,000 pmoles/min/ug.
Formulation	Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.