Name: Recombinant Human Carbonic Anhydrase X / CA10 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0560
Availability: InStock

Recombinant Human Carbonic Anhydrase X / CA10 Protein (His Tag)

Catalog No.: BLPSN-0560

Product Overview

Tag	His
Host Species	Human
Accession	NP_001076002.1
Synonym	CA-RPX, CA10, CARPX, HUCEP-15
Background	Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA1, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.
Description	A DNA sequence encoding the human CARPX (NP_001076002.1) (Met 1-Asn 300) was expressed with a C-terminal His tag.
Source	HEK293
Predicted N Terminal	Gln 22
AA Sequence	Met 1-Asn 300
Molecular Weight	The recombinant human CARPX consists of 291 a.a. and has a predicted molecular mass of 33 kDa. rhCARPX migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its esterase activity. The specific activity is >10 pmoles/min/ug, as measured with 1 mM 4-Nitrophenyl acetate and 5 ug enzyme at 400 nm in 100 uL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.