Recombinant Human Calreticulin Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0527
Recombinant Human Calreticulin Protein (His Tag)

Recombinant Human Calreticulin Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0527
Catalog No.: BLPSN-0527

Product Overview

Tag His
Host Species Human
Accession P27797
Synonym cC1qR, CRT, HEL-S-99n, RO, SSA
Background Calreticulin is a multifunctional protein. It acts as a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction), rendering it inactive. The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum, calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Therefore, calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.
Description A DNA sequence encoding the human CALR (P27797) (Met1-Ala413) was expressed with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Glu 18
AA Sequence Met1-Ala413
Molecular Weight The recombinant human CALR consists of 407 a.a. and predicts a molecular mass of 47.4 KDa. It migrates as an approximately 58 KDa band in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recently viewed