Recombinant Human Cadherin 8 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0502
Recombinant Human Cadherin 8 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0502
Catalog No.: BLPSN-0502
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_001787.2 |
| Synonym | Nbla04261 |
| Background | Cadherins are integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Type I cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of five subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherin 8, also known as CDH 8, is a type II classical cadherin belonging to the cadherin superfamily. As mainly expressed in brain, CDH8 is found in certain nerve cell lines, such as retinoblasts, glioma cells and neuroblasts, and is putatively involved in synaptic adhesion, axon outgrowth and guidance. Human Cadherin 8 is a 799 amino acid single-pass type Itransmembrane protein with a putative 29 aa signal sequence, and a 32 aa propeptide, a 56 aa mature extracellular domain, a 21 aa transmembrane domain and a 157 aa cytoplasmic domain. The human, mouse and rat proteins share approximately 98% homology. |
| Description | A DNA sequence encoding the extracellular domain of human CDH8 (NP_001787.2) (Met 1-Met 621) was expressed with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Ala 30 |
| AA Sequence | Met 1-Met 621 |
| Molecular Weight | The secreted pro form of human CDH8 consists of 603 a.a. and has a predicted molecular mass of 66.6 kDa. As a result of glycosylation, it migrates with an apparent molecular mass of 85-90 kDa in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
