Recombinant Human C1QB Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0479
Recombinant Human C1QB Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0479
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | NP_000482.3 |
Synonym | C1QB |
Background | Complement Component 1, q subcomponent (C1q) associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Southern blot analysis of chromosomal DNA from vertebrate species demonstrated highest similarity between the C1qB genes, followed by C1qC and finally C1qA. Sequence comparison of C1q from three different species have shown that the B chains have the strongest similarity. C1q was already present at embryonic day 14 (E14) and showed little change in abundance through six weeks postnatal. At E16, C1qB mRNA was present at high abundance in putative microglia/macrophages in cortical marginal and intermediate zones, and hippocampal analge. |
Description | A DNA sequence encoding the human C1QB (NP_000482.3) precursor (Met 1-Ala 253) was expressed, with a carboxy-terminal His tag. |
Source | Baculovirus-Insect Cells |
Predicted N Terminal | Gln 28 |
AA Sequence | Met 1-Ala 253 |
Molecular Weight | The recombinant human C1QB consists of 237 a.a. with the predicted molecular mass of 25 kDa. As a result of glycosylation, rhC1QB migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions. |
Purity | >94% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM TCEP, 10% glycerol, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |