Recombinant Human C16ORF53 Protein

Name: Recombinant Human C16ORF53 Protein
Brand: Beta LifeScience
SKU: BL-2905PS
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Synonym	PAXIP1-associated protein 1, PTIP-associated protein 1, PA1, C16orf53, GAS.
Background	C16ORF53 is a component of a Set1-like multiprotein histone methyltransferase complex. C16ORF53 interacts with PAXIP1/PTIP; this interaction is direct and is necessary for the association with the rest of the PTIP complex. The C16ORF53 protein has a crucial role in maintaining genome stability, condensation of chromatin and progression through mitosis.
Description	C16ORF53 expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 274a.a. (1-254 a.a.) and having a molecular weight of 29.9kDa (Molecular weight on SDS-PAGE will appear higher).C16ORF53 is fused to a 20a.a. His-tag at N-terminus and purified by unique purification methods.
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MSLARGHGDT AASTAAPLSE EGEVTSGLQA LAVEDTGGPS ASAGKAEDEG EGGREETERE GSGGEEAQGE VPSAGGEEPA EEDSEDWCVP CSDEEVELPA DGQPWMPPPS EIQRLYELLA AHGTLELQAE ILPRRPPTPE AQSEEERSDE EPEAKEEEEE KPHMPTEFDF DDEPVTPKDS LIDRRRTPGS SARSQKREAR LDKVLSDMKR HKKLEEQILR TGRDLFSLDS EDPSPASPPL RSSGSSLFPR QRKY.
Purity	>85.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	C16ORF53 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	C16ORF53 Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.