Recombinant Human Bikunin Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0405
Recombinant Human Bikunin Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0405
Catalog No.: BLPSN-0405
Product Overview
| Tag | Fc |
| Host Species | Human |
| Accession | NP_001624.1 |
| Synonym | A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI |
| Background | The AMBP [A1M (alpha1-microglobulin)/bikunin precursor] gene encodes two plasma glycoproteins: A1M, an immunosuppressive lipocalin, and bikunin, a member of plasma serine proteinase inhibitor family with prototypical Kunitz-type domain. Although previously believed to be constitutively expressed exclusively in liver, the present study demonstrates the induction of this gene by oxalate in porcine proximal tubular LLC-PK1 cells and rat kidney. In liver, the precursor protein is cleaved in the Golgi network by a furin-like enzyme to release constituent proteins, which undergo glycosylation before their export from the cell. In the renal tubular cells, A1M and bikunin co-precipitate, indicating lack of cleavage of the precursor protein. As the expression of the AMBP gene is regulated by A1M-specific cis elements and transcription factors, A1M protein was studied as a representative of AMBP gene expression in renal cells. The alpha(1)-microglobulin/bikunin precursor (AMBP) gene, and its two protein products were studied in mouse embryos of 8.5-15.5 days of embryonic development by in situ hybridization and immunohistochemistry. AMBP mRNA is strongly transcribed in liver parenchyma, pancreas, and intestine epithelium. Sites of weaker expression are the vessels of the umbilical cord, the developing vertebral bodies, and kidney. The alpha(1)-microglobulin and bikunin proteins are accordingly present in developing hepatocytes, pancreas, kidney, and gut. However, additional sites of protein distribution were found that do not correlate to mRNA localization: alpha(1)-microglobulin was found in myocytes and bikunin in cardiac muscle, nervous system microvasculature, and connective tissue |
| Description | A DNA sequence encoding the alpha-1-microglobulin of human AMBP (NP_001624.1) (Met1-Val203) was expressed with the Fc region of mouse IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Gly 20 |
| AA Sequence | Met1-Val203 |
| Molecular Weight | The recombinant alpha-1-microglobulin of human AMBP consists 418 a.a. and predicts a molecular mass of 47.2 kDa. |
| Purity | >(14.7+83.9)% as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
