Recombinant Human BCMA Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0377
Recombinant Human BCMA Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0377
Catalog No.: BLPSN-0377
Product Overview
| Tag | His&Fc |
| Host Species | Human |
| Accession | NP_001183.2 |
| Synonym | BCM, BCMA, CD269, TNFRSF13A |
| Background | Tumor necrosis factor receptor superfamily, member 17 (TNFRSF17), also known as B cell maturation antigen (BCMA) or CD269 antigen, is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13BBAFF), and to lead to NF-kappaB and MAPK8/JNK activation. TNFRSF17/BCMA/CD269 also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. TNFRSF17/BCMA/CD269 is a receptor for TALL-1 and BCMA activates NF-kappaB through a TRAF5-, TRAF6-, NIK-, and IKK-dependent pathway. The identification of TNFRSF17 as a NF-kappaB-activating receptor for TALL-1 suggests molecular targets for drug development against certain immunodeficient or autoimmune diseases. TNFRSF17/BCMA is a target of donor B-cell immunity in patients with myeloma who respond to DLI. Antibody responses to cell-surface BCMA may contribute directly to tumor rejection in vivo. |
| Description | A DNA sequence encoding the extracellular domain of human TNFRSF17 (NP_001183.2) (Met 1-Ala 54) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus and a signal peptide at the N-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Met 1 |
| AA Sequence | Met 1-Ala 54 |
| Molecular Weight | The recombinant human TNFRSF17/Fc is a disulfide-linked homodimer. The reduced monomer comprises 302 a.a. after removal of the signal peptide and has a predicted molecular mass of 34 kDa. As a result of glycosylation, the apparent molecular mass of rh TNFRSF17/Fc monomer is approximately 40 kDa in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized recombinant human BAFF at 1 ug/ml (100 ul/well) can bind human TNFRSF17. The EC50 of human TNFRSF17 is 0.07 ug/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
