Recombinant Human BCL2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0367
Recombinant Human BCL2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0367
Catalog No.: BLPSN-0367
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P10415 |
| Synonym | Bcl-2, PPP1R50 |
| Background | BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
| Description | A DNA sequence encoding the human BCL2 isoform 1 (P10415-) (Met 1-Asp 211) was expressed, with a His tag at the C-terminus. |
| Source | E.coli |
| Predicted N Terminal | Met 1 |
| AA Sequence | Met 1-Asp 211 |
| Molecular Weight | The recombinant human BCL2 consisting of 221 a.a. and has a calculated molecular mass of 24.7 kDa. It migrates as an approximately 32 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human BCL2-His at 10 ug/ml (100 ul/well) can bind biotinylated mouse BCL2L1-His, The EC50 of biotinylated mouse BCL2L1-His is 0.07-0.15 ug/ml. |
| Formulation | Lyophilized from sterile 50mM Tris, 20% glycerol, 100mM Arg, pH 8.5. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
