Recombinant Human Bcl-XL Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0372
Recombinant Human Bcl-XL Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0372
Catalog No.: BLPSN-0372
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_612815.1 |
| Synonym | Bcl-X, bcl-xL, BCL-XL/S, bcl-xS, BCL2L, BCLX, BCLXL, BCLXS, PPP1R52 |
| Background | B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1) , belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic alpha-helices, which are surrounded by amphipathic helices. A 6-residue loop connecting helices alphal and alpha2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death. |
| Description | A DNA sequence encoding the human BCL2L1 isoform 1 (NP_612815.1) (Met 1-Arg 212) was fused with a His tag at the C-terminus. |
| Source | E.coli |
| Predicted N Terminal | Met |
| AA Sequence | Met 1-Arg 212 |
| Molecular Weight | The recombinant human BCL2L1 consisting of 222 a.a. and has a calculated molecular mass of 25.2 kDa. It migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | 1. Measured by its binding ability in a functional ELISA.2. Immobilized human BID at 10 ug/mL (100 ul/well) can bind- biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.1 ng/mL.3. Immobilized mouse BID at 10 ug/mL (100 ul/well) can bind- biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.01 ng/mL. |
| Formulation | Lyophilized from sterile 20mM Tris, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
