Recombinant Human BCCIP Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0366
Recombinant Human BCCIP Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0366
Catalog No.: BLPSN-0366
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | AAH09771.1 |
| Synonym | TOK-1, TOK1 |
| Background | BCCIP was originally identified as a BRCA2 and CDKN1A interacting protein that has been implicated in maintenance of genomic stability, cell cycle regulation, and microtubule dynamics. The secondary genetic alternations may overcome the progression suppression imposed by BCCIP deficiency through a synthetic viability mechanism. Knockdown of YY1 inhibited the binding of BCCIP itself at BCCIP promoter region proximal to TSS, demonstrating that transcriptional regulation of the YY1 on BCCIP can be modulated by BCCIP itself in a YY1-dependent fashion. BCCIP (BRCA2 and CDKN1A interacting protein) plays a critical role in maintaining the critical functions of p53 in tumor suppression and response to therapy. Celecoxib affects the functions of p53 and inhibits the recovery from the irradiation-induced injury by up-regulating the expression of BCCIP, and subsequently regulates the expressions of genes such as p21 and Cyclin B1 to enhance the radiosensitivity of HCT116 cells in a COX-2 independent manner. |
| Description | A DNA sequence encoding the human BCCIP (AAH09771.1) (Met1-Val314) was expressed with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met1-Val314 |
| Molecular Weight | The recombinant human BCCIP/GST chimera consists of 551 a.a. and has a calculated molecular mass of 64 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
