Recombinant Human Aspartate Aminotransferase / ASTQTL1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0283
Recombinant Human Aspartate Aminotransferase / ASTQTL1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0283
Catalog No.: BLPSN-0283
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P17174 |
| Synonym | ASTQTL1, cAspAT, cCAT, GIG18 |
| Background | Aspartate aminotransferase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, aspartate aminotransferase and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. There is a rare in-frame deletion in aspartate aminotransferase gene, which inactivates cytosolic aspartate aminotransferase(cAST) enzyme in the Old Order Amish. This may help to understand structure and function of the enzyme and would be useful for predicting serum aspartate AST levels. |
| Description | A DNA sequence encoding the mature form of human GOT1 (P17174) (Met1-Gln413) was expressed with a His tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | His |
| AA Sequence | Met1-Gln413 |
| Molecular Weight | The recombinant human GOT1 consists of 428 a.a. and predicts a molecular mass of 48.1 KDa. It migrates as an approximately 36-45 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
