Name: Recombinant Human ARG1 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0254
Availability: InStock

Recombinant Human ARG1 Protein (His Tag)

Catalog No.: BLPSN-0254

Product Overview

Tag	His
Host Species	Human
Accession	P05089
Synonym	ARG1
Background	Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.
Description	A DNA sequence encoding the human ARG1 isoform 1 (P05089-1) (Met 1-Lys 322) was fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Met 1
AA Sequence	Met 1-Lys 322
Molecular Weight	The secreted recombinant human ARG1 consists of 333 a.a. and has a calculated molecular mass of 36.2 kDa. The apparent molecular mass of the protein is approximately 40 kDa in SDS-PAGE under reducing conditions.
Purity	>90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by the production of urea during the hydrolysis of arginine.The specific activity is >35,000 pmoles/min/ug.
Formulation	Lyophilized from sterile 20mM Tris, 150mM NaCl, 20% Glycerol, 1mM DTT, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.