Recombinant Human Apolipoprotein A-I Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-0238
Recombinant Human Apolipoprotein A-I Protein (Fc Tag)

Recombinant Human Apolipoprotein A-I Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-0238

Product Overview

Tag Fc
Host Species Human
Accession CAA26097.1
Synonym Apolipoprotein A-I
Background Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.
Description A DNA sequence encoding the pro-form of human APOA1 (CAA26097.1) (Met 1-Gln 267) was fused with Fc region of human IgG1 at the C-terminus.
Source HEK293
Predicted N Terminal Asp 25
AA Sequence Met 1-Gln 267
Molecular Weight The recombinant human APOA1/Fc is a disulfide-linked homodimeric protein.The reduced monomer consists of 481 a.a. migrates as approximately 55 kDa band in SDS-PAGE under reducing conditions as predicted.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1. Measured by its binding ability in a functional ELISA.2. Immobilized Human ApoAI at 10 ug/mL (100 ul/well) can bind-  biotinylated human SCARB1, The EC50 of biotinylated human SCARB1 is 0.37 ug/mL.
Formulation Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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