Recombinant Human ANAPC13 Protein

Beta LifeScience SKU/CAT #: BL-3634PS
Recombinant Human ANAPC13 Protein

Recombinant Human ANAPC13 Protein

Beta LifeScience SKU/CAT #: BL-3634PS
Catalog No.: BL-3634PS

Product Overview

Tag N/A
Host Species Human
Synonym Anaphase-promoting complex subunit 13, APC13, Cylosome subunit 13, ANAPC13, SWM1.
Background Anaphase-promoting complex subunit 13 (ANAPC13) is a component of the anaphase promoting complex, which is a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. The ANAPC13 protein is evolutionarily conserved and is essential for the integrity and ubiquitin ligase activity of the anaphase promoting complex.
Description ANAPC13 Human Recombinant expressed in E.Coli is a single, non-glycosylated, polypeptide chain containing 89a.a. (1-74) and having a molecular weight of 10kDa (Molecular weight on SDS-PAGE will appear higher).ANAPC13 protein is fused to a 15a.a. T7-tag at N-terminus and is purified by standard chromatography.
Source E.coli
AA Sequence MASMTGGQQM GRGSHMDSEV QRDGRILDLI DDAWREDKLP YEDVAIPLNE LPEPEQDNGG TTESVKEQEM KWTDLALQYL HENVPPIGN.
Purity >90.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The ANAPC13 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 1mM DTT and 0.1M NaCl.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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