Name: Recombinant Human ALK-1 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0138
Availability: InStock

Recombinant Human ALK-1 Protein (His Tag)

Catalog No.: BLPSN-0138

Product Overview

Tag	His
Host Species	Human
Accession	NP_000011.2
Synonym	ACVRLK1, ALK-1, ALK1, HHT, HHT2, ORW2, SKR3, TSR-I
Background	Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.
Description	A DNA sequence encoding the the extracellular domain of human ALK1 (NP_000011.2) (Met 1-Gln 118) was fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Asp 22
AA Sequence	Met 1-Gln 118
Molecular Weight	The recombinant human ALK1 comprises 108 a.a. and has a predicted molecular mass of 12.3 kDa. As a result of glycosylation, rhALK1 migrates as an approximately 27 kDa protein in SDS-PAGE under reducing conditions.
Purity	>92% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953.The ED50 for this effect is typically 50-200 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.