Recombinant Human AIM2 Protein (GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-0112
Recombinant Human AIM2 Protein (GST Tag)

Recombinant Human AIM2 Protein (GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-0112

Product Overview

Tag GST
Host Species Human
Accession NP_004824.1
Synonym PYHIN4
Background AIM2, Absent In Melanoma 2 is a member of the interferon-inducible HIN-2 protein family that contains an amino-terminal pyrin domain and a carboxy-terminal oligonucleotide/oligosaccharide-binding domain, senses cytoplasmic DNA by means of its oligonucleotide/oligosaccharide-binding domain and interacts with ASC (apoptosis-associated speck-like protein containing a CARD) through its pyrin domain to activate caspase-1. In response to foreign cytoplasmic DNA, AIM2 forms an inflammasome, resulting in caspase activation in inflammatory cells. It had been pointed to a role of AIM2 function in both inflammation and cancer. AIM-2 antigen is expressed in a wide variety of tumor types, including neuroectodermal tumors, as well as breast, ovarian and colon carcinomas. AIM-2 could be used as a tumor antigen target for monitoring vaccine trials or to develop antigen specific active immunotherapy for glioma patients.
Description A DNA sequence encoding the human AIM2 (NP_004824.1) (Met 1-Thr 343) was fused with the GST tag at the N-terminus.
Source Baculovirus-Insect Cells
Predicted N Terminal Met
AA Sequence Met 1-Thr 343
Molecular Weight The recombinant human AIM2/GST chimera consists of 567 a.a. and predicts a molecular mass of 65.2 kDa as estimated in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 10-40ug/ml.
Formulation Lyophilized from sterile 50mM Tris, 1M NaCl, 0.5mM PMSF, 5mM GSH, pH 8.0.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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