Recombinant Human Adenosine Deaminase / ADA Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0090
Recombinant Human Adenosine Deaminase / ADA Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0090
Catalog No.: BLPSN-0090
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P00813 |
| Synonym | ADA, ADA1 |
| Background | Adenosine Desaminase (ADA) deficiency, is a purine metabolic disorder that cause severe combined immunodeficiency (SCID) due to the accumulation of toxic metabolites that primarily affects development, differentiation and function of T and B lymphocytes. Adenosine deaminase is a polymorphic enzyme that has an important role in immune functions and in the regulation of intracellular and extracellular concentrations of adenosine and adenosine receptor activity. ADA activity might be considered as a useful diagnostic tool among the other markers in these diseases. Genetic variability of ADA activity may have, therefore, an important role in resistance to malaria. Adenosine Deaminase (ADA) deficiency is an autosomal recessive variant of severe combined immunodeficiency (SCID) caused by systemic accumulation of ADA substrates. |
| Description | A DNA sequence encoding the human ADA (P00813) (Met1-Leu363) was expressed with a C-terminal His tag. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met1-Leu363 |
| Molecular Weight | The secreted recombinant human ADA consists of 373 a.a. and predicts a molecular mass of 42.1 KDa. The apparent molecular mass of the protein is approximately 44 KDa in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
