Recombinant Human ACVR2A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0070
Recombinant Human ACVR2A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0070
Catalog No.: BLPSN-0070
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_001607.1 |
| Synonym | ACTRII, ACVR2 |
| Background | ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98% identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers). Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways. |
| Description | A DNA sequence encoding the extracellular domain of human ACVR2A (NP_001607.1) (Met 1-Pro 134) was expressed, with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Ala 20 |
| AA Sequence | Met 1-Pro 134 |
| Molecular Weight | The recombinant human ACVR2A comprises 126 a.a. and predicts a molecular mass of 14.9 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 35-40 kDa in SDS-PAGE under reducing conditions. |
| Purity | >97% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.The ED50 for this effect is typically 0.4-3 µg/mL in the presence of 10 ng/mL recombinant Activin A. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
