Recombinant Human ACTN1 Protein

Beta LifeScience SKU/CAT #: BL-3619PS
Recombinant Human ACTN1 Protein

Recombinant Human ACTN1 Protein

Beta LifeScience SKU/CAT #: BL-3619PS
Catalog No.: BL-3619PS

Product Overview

Tag N/A
Host Species Human
Synonym ACTN1, Actinin, Alpha 1, Alpha-Actinin CytoskeletalIsoform, F-Actin Cross-Linking Protein, Non-Muscle Alpha-Actinin-1, BDPLT15,Actinin 1 Smooth Muscle, Alpha-Actinin-1.
Background ACTN1 encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
Description ACTN1 Human Recombinant expressed in E.Coli is a single,non-glycosylated polypeptide chain containing 274a.a. (1-249 a.a) andhaving a molecular weight of 31.4kDa. ACTN1 is fused to a 25a.a. His-tag at N-terminusand purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHHSSGLVPRGSH MGSEFMDHYD SQQTNDYMQP EEDWDRDLLL DPAWEKQQRKTFTAWCNSHL RKAGTQIENI EEDFRDGLKL MLLLEVISGE RLAKPERGKM RVHKISNVNK ALDFIASKGVKLVSIGAEEI VDGNVKMTLG MIWTIILRFA IQDISVEETS AKEGLLLWCQ RKTAPYKNVN IQNFHISWKDGLGFCALIHR HRPELIDYGK LRKDDPLTNL NTAFDVAEKY LDIPKMLDAE DIVGTARPDE KAIMTYVSSF YHAF.
Purity Greaterthan 95.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation ACTN1 protein solution (1mg/ml) containing PhosphateBuffered Saline (pH7.4) and 10% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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