Recombinant Human ABHD4 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0032
Recombinant Human ABHD4 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0032
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_071343.2 |
| Synonym | ABH4 |
| Background | Abhydrolase domain containing 4 (ABHD4), also known as alpha/beta-hydrolase 4 (ABH4) , or lyso-N-acylphosphatidylethanolamine lipase, which belongs to the ABHD4/ABHD5 subfamily of peptidase S33 family. Abhydrolase domain containing (ABHD) gene was a small group belongs to alpha/beta hydrolase superfamily. Known members of this group are all found to be involved in important biochemical processes and related to various diseases. The alpha/beta-hydrolase 4 (ABH4) is a lysophospholipase/phospholipase B that selectively hydrolyzes N-acyl phosphatidylethanolamines (NAPEs) and lysoNAPEs. ABH4 accepts lysoNAPEs bearing both saturated and polyunsaturated N-acyl chains as substrates and displays a distribution that closely mirrors lysoNAPE-lipase activity in mouse tissues. The existence of an NAPE-PLD-independent route for NAE biosynthesis and suggest that ABH4 plays a role in this metabolic pathway by acting as a (lyso)NAPE-selective lipase. |
| Description | A DNA sequence encoding the full length of human ABHD4 (NP_071343.2) (Met 1-Asp 342) was expressed, with a His tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | His |
| AA Sequence | Met 1-Asp 342 |
| Molecular Weight | The recombinant human ABHD4 consists of 360 a.a. and predicts a molecular mass of 41 kDa. It migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >82% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
