Name: Recombinant Cynomolgus Her2 / ERBB2 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2392
Availability: InStock

Recombinant Cynomolgus Her2 / ERBB2 Protein (His Tag)

Catalog No.: BLPSN-2392

Product Overview

Tag	His
Host Species	Cynomolgus
Accession	EHH58073.1
Synonym	ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, erb-b2 receptor tyrosine kinase 2
Background	Receptor tyrosine-protein kinase erbB-2, also known as CD340 (cluster of differentiation 340), proto-oncogene Neu, Erbb2 (rodent), or ERBB2 (human), is a protein that in humans is encoded by the ERBB2 gene. ERBB is abbreviated from erythroblastic oncogene B, a gene isolated from avian genome. It is also frequently called HER2 (from human epidermal growth factor receptor 2) or HER2/neu. ERBB2, commonly referred to as HER2, is amplified and/or overexpressed in 20-30% of invasive breast carcinomas. HER2-positive breast cancer is treated in a separate manner from other subtypes of breast cancer and commonly presents as more aggressive disease. Metastatic HER2-positive breast cancer is now commonly treated with HER2-targeted therapy. Apart from being amplified/overexpressed, ERBB2 activating mutations have been shown to have clinical importance in HER2-negative breast cancer. These mutations have shown sensitivity to the tyrosine kinase inhibitor neratinib, and highlight the importance of clinical sequencing efforts in treating breast cancer.
Description	A DNA sequence encoding the cynomolgus ERBB2 (Met1-Thr652) [similar to Macaca fascicularis ERBB2 (EHH58073.1)] was expressed with a Histag atthe C-terminus.
Source	HEK293
Predicted N Terminal	Thr 23
AA Sequence	Met1-Thr652
Molecular Weight	The recombinant cynomolgus ERBB2 comprises 641 amino acids and has a calculated molecular mass of 70.8 KDa. The apparent molecular mass of it is approximately 80-105 KDa in SDS-PAGE under reducing conditions.
Purity	>90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its binding ability in a functional ELISA.Immobilized Cynomolgus ERBB2-His at 10 ug/ml (100 ul/well) can bind Herceptin, The EC50 of Herceptin is 16.4-38.4 ng/ml.
Formulation	Recombinant Cynomolgus Her2 / ERBB2 Protein (His Tag) was lyophilized from sterile PBS, pH 7.4.
Stability	Recombinant Cynomolgus Her2 / ERBB2 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.