|description||MagicScript II cDNA First Strand Synthesis Kit features two optimized mixes, MagicScript II Enzyme Mix and MagicScript II Reaction Mix. The enzyme mix combines MagicScript II Reverse Transcriptase and RNase Inhibitor, while the reaction mix contains an optimized buffer. MagicScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 50°C, providing higher specificity and higher yield of cDNA.
The kit also provides two optimized primers for reverse transcription and nuclease-free water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The first strand cDNA product generated is up to 10 kb.
|applications||cDNA Synthesis; RT-PCR & cDNA Synthesis; Reverse Transcription (cDNA Synthesis)|
|size||50 rxn / 100 rxn|
|components||* MagicScript II Reaction Mix (2X)
* MagicScript II Enzyme Mix (10X)
* Oligo d(T)23VN (50 uM)
* dNTPs (10 mM)
* Nuclease-free H2O
* Random Primer Mix (60 uM)
|usage||For Research Use Only|
|storage||Store the MagicScript II First Strand cDNA Synthesis Kit at -20°C. Please avoid repeated freeze-thaw cycles.|