IL12B Protein, Marmoset (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2664
IL12B Protein, Marmoset (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2664
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Product Overview
Tag | Fc |
Host Species | Marmoset |
Accession | F7IQ84 |
Background | Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p4) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 4 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. IL12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. IL12B/IL-12B associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
Description | A DNA sequence encoding the marmoset IL12B (F7IQ84) (Met1-Asn328) was expressed with the Fc region of human IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Ile 23 |
AA Sequence | Met1-Asn328 |
Molecular Weight | The recombinant marmoset IL12B is a disulfide-linked homodimer. The reduced monomer comprises 547 a.a. and has a calculated molecular mass of 61.9 KDa.The apparent molecular mass of the protein is approximately 66 KDa in SDS-PAGE. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured by its binding ability in a functional ELISA.2.Immobilized marmoset IL12B-Fc at 10 ug/mL (100 uL/well) can bind biotinylated human IL12RB1-His. The EC50 of human IL12RB1-His is 0.66-1.53 ug/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |